Voltammetric Experiments for Bioanalytical Chemistry


1. "Determination of Anionic Surfactants Using Atomic Absorption Spectrometry and Anodic Stripping Voltammetry" John, Richard; Lord, Daniel. J. Chem. Educ. 1999 76 1256. (September 1999)
An experiment has been developed for our undergraduate analyticalchemistry course that demonstrates the indirect analysis of anionic surfactants by techniques normally associated with metal ion determination; that is, atomic absorption spectroscopy (AAS) and anodic stripping voltammetry (ASV). The method involves the formation of an extractable complex between the synthetic surfactant anion and the bis(ethylenediamine)diaqua copper(II) cation. This complex is extracted into chloroform and then back-extracted into dilute acid. The resulting Cu(II) ions are determined by AAS and ASV. Students are required to determine the concentration of a pre-prepared "unknown" anionic surfactant solution and to collect and analyze a real sample of their choice. After the two extraction processes, students typically obtain close to 100% analytical recovery. Correlation between student AAS and ASV results is very good, indicating that any errors that occur probably result from their technique (dilutions, extractions, preparation of standards, etc.) rather than from the end analyses.

2. "Using Cyclic Voltammetry and Molecular Modeling To Determine Substituent Effects in the One-Electron Reduction of Benzoquinones" Heffner, Janell E.; Raber, Jeffrey C.; Moe, Owen A., Jr.; Wigal, Carl T. J. Chem. Educ. 1998 75 365. (March 1998)
An experiment has been developed to teach the principles of molecular luminescence spectroscopy. This laboratory experiment is designed for upper-level undergraduates as a less toxic alternative to current fluorescence experiments. It combines elements of physical and inorganic as well as analytical chemistry. The experiment can be performed on a variety of rudimentary fluorescence instrumentation and still give good analytical figures of merit. The object of the experiment is to measure the luminescent enhancement that is achieved when a lanthanide such as Eu(III) or Tb(III) is complexed with appropriate organic ligands, in this case 2,6 pyridinedicarboxylic acid. The importance of pH on metal ion coordination is also explored via luminescence intensity. This approach provides several advantages over current luminescence experiments. These advantages include limited toxicity and flammability of the chemicals involved, a large luminescence linear dynamic range, and low detection limits (parts per trillion). These low detection limits, achieved using modest equipment, allow the determination of the europium concentration in a variety of samples, such as tap water. The narrow lanthanide luminescent bands also permit incorporation of qualitative analysis of a mixture of lanthanides.

3. "The use of cyclic voltammetry in the study of the chemistry of metal-carbonyls: An introductory experiment" Carriedo, Gabino A. . J.Chem. Educ. 1988 65 1020.

4. "A cyclic voltammetry experiment using a mercury electrode" Brillas, Enrique; Garrido, Jose A.; Rodriquez, Rosa M.; Domenech, Javier. J. Chem. Educ. 1987 64 189.

5. "Voltammetry as a model for teaching chemical instrumentation" Gunasingham, H.; Ang, K. P. J. Chem. Educ. 1985 62 610.

6. "A cyclic voltammetry experiment for the instrumental analysis laboratory" Baldwin, Richard P.; Ravichandran, K.; Johnson, Ronda K. J. Chem. Educ. 1984 61 820.

7. "Anodic stripping voltammetry: an instrumental analysis experiment" Wang, Joseph. J. Chem. Educ. 1983 60 1074.

8. "Cyclic voltammetry experiment" Van Benschoten, James J.; Lewis, Jane Y.; Heineman, William R.; Roston, Daryl A.; Kissinger, Peter T. J. Chem. Educ. 1983 60 772.

2. "The effect of complex formation upon the redox potentials of metallic ions: Cyclic voltammetry experiments" Ibanez, Jorge G.; Gonzalez, Ignacio; Cardenas, Marco A. J. Chem. Educ. 1988 65 173.

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