HPLC Experiments Suitable for Bioanalytical Chemistry Labs

 

Peptides & Proteins

Braco, Lorenzo; Ba­-, M. Carmen; Abad, Concepci-n. Monitoring self-association of a hydrophobic peptide with high performance liquid chromatography: An undergraduate kinetic experiment using the antibiotic gramicidin A (MODLAB). J. Chem. Educ. 1992 69 A113.

Clapp, Charles H.; Swan, James S.; Poechmann, James L. Identification of amino acids in unknown dipeptides: A derivatization with 9-fluorenylmethyl chloroformate and HPLC (MODLAB). J. Chem. Educ. 1992 69 A122.

Kenigsberg, Paul A.; Blanke, Steven R.; Hager, Lowell P. Isolation of the active site of cytochrome c by reverse-phase high-performance liquid chromatography. J. Chem. Educ. 1990 67 177.

Richardson, W. S., III; Burns, L. HPLC of the polypeptides in a hydrolyzate of egg white lysozyme: An experiment for the undergraduate biochemistry laboratory. J. Chem. Educ. 1988 65 162.

Vestling, Martha M. A peptide HPLC experiment for biochemistry laboratory. J. Chem. Educ. 1991 68 72.

Vestling, Martha M. Insulin: HPLC mapping of protease digestion products: A biochemistry laboratory experiment. J. Chem. Educ. 1991 68 958.

Wietstock, Steven M. DNA Composition Analysis by Nuclease Digestion and HPLC J. Chem. Educ. 1995 72 950. (October 1995)Vestling, Martha M. Insulin: HPLC mapping of protease digestion products: A biochemistry laboratory experiment. J. Chem. Educ. 1991 68 958.

 

Pharmaceuticals

Beaver, Rodney W.; Bunch, John E.; Jones, Louis A. Qualitative analysis of analgesic tablets: an experiment employing high-pressure liquid chromatography. J. Chem. Educ. 1983 60 1000.

Ferguson, Glenda K. Quantitative HPLC Analysis of a Psychotherapeutic Medication: Simultaneous Determination of Amitriptyline Hydrochloride and Perphenazine J. Chem. Educ. 1998 75 1615. (December 1998)

Glenda K. Ferguson Quantitative HPLC Analysis of an Analgesic/Caffeine Formulation: Determination of Caffeine J. Chem. Educ. 1998 75 467. (April 1998)

Kagel, R. A.; Farwell, S. O. Analysis of currently available analgesic tablets by modern liquid chromatography: an undergraduate laboratory introduction to HPLC. J. Chem. Educ. 1983 60 163.

Krumpolc, Miroslav. Microscale resolution of racemic 1-phenylethylamine and chiral high performance liquid chromatography (HPLC) in undergraduate chemistry (ML). J. Chem. Educ. 1991 68 A176.

Haddad, Paul; Hutchins, Stephen; Tuffy, Michael. High performance liquid chromatography of some analgesic compounds: an instrumental analysis experiment. J. Chem. Educ. 1983 60 166.

Mueller, Brian L.; Potts, Lawrence W. HPLC analysis of an asthma medication. J. Chem. Educ. 1988 65 905.

 

Vitamins

Munari, Marina; Miurin, Maria; Goi, Gianfranco. Didactic application to riboflavin HPLC analysis: A laboratory experiment. J. Chem. Educ. 1991 68 78.

Goodner, David E. Analysis of vitamin C by high-pressure liquid chromatography. J. Chem. Educ. 1987 64 187.

Bohman, Ove; Engdahl, Kjell-Ake; Johnsson, Hakan. High performance liquid chromatography of vitamin A. J. Chem. Educ. 1982 59 251.

 

Food & Beverage

Betts, Thomas A. Pungency Quantitation of Hot Pepper Sauces Using HPLC J. Chem. Educ. 1999 76 240. (February 1999)

Bidlingmeyer, Brian A.; Schmitz, Stefan. The analysis of artifical sweeteners and additives in beverages by HPLC: An undergraduate experiment (The modern student laboratory). J. Chem. Educ. 1991 68 A195.

DiNunzio, James E. Determination of caffeine in beverages by high performance liquid chromatography. J. Chem. Educ. 1985 62 446.

Delaney, Michael F.; Pasko, Kathleen M.; Mauro, David M.; Gsell, Diane S.; Korologos, Philip C.; Morawski, John; Krolikowski, Linda J.; Warren, F. Vincent, Jr. Determination of aspartame, caffeine, saccharin, and benzoic acid in beverages by high performance liquid chromatography: An undergraduate analytical chemistry experiment. J. Chem. Educ. 1985 62 618.

Luo, Peifang; Luo, Matthew Z.; Baldwin, Richard P. Determination of sugars in food products: Using HPLC and electrochemical detection at a Cu electrode. J. Chem. Educ. 1993 70 679.

Strohl, Arthur N. A study of colas: An HPLC experiment. J. Chem. Educ. 1985 62 447.

 

Plants

Silveira, Augustine, Jr.; Koehler, Jerry A.; Beadel, Edward F., Jr.; Monroe, Pearle A. HPLC analysis of chlorophyll a, chlorophyll b, and beta-carotene in collard greens. J. Chem. Educ. 1984 61 264.

 

Clinical

Bastian, Douglas W.; Miller, Rochelle L.; Halline, Allan G.; Senftleber, Fred C.; Veening, Hans. High performance liquid chromatography of urinary compounds. An undergraduate experiment. J. Chem. Educ. 1977 54 766.

 

Method Optimization

Harvey, David T.; Byerly, Shannon; Bowman, Amy; Tomlin, Jeff. Optimization of HPLC and GC separations using response surfaces: Three experiments for the instrumental analysis laboratory. J. Chem. Educ. 1991 68 162.

Remcho, Vincent T.; McNair, Harold M.; Rasmussen, Henrik T. HPLC method development with the photodiode array detector: A laboratory experiment (MODLAB). J. Chem. Educ. 1992 69 A117.

 

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